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Fig. 1. Dependence of ErbB2 homoassociation on local parameters. SKBR-3 breast tumor cells were labeled with FITC-4D5 and Cy3-4D5 against ErbB2 to measure the homoassociation of ErbB2; ErbB3 was labeled with Cy5-tagged secondary Fab following labeling with H3.90.6. The homoassociation of ErbB2 was measured with the donor pbFRET method, and it was correlated with the local density of ErbB2 and ErbB3 on a pixel-by-pixel basis. The average FRET efficiency for ErbB2 homoassociation is plotted as a function of the local density of ErbB2 and ErbB3.





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