Fig. 5. Relationship between caveolin, GM1-enriched domains and ErbB2 clusters. (A) Quiescent SKBR-3 cells were labeled with FITC-CTX-B (green channel) and with anti-caveolin antibody sc-894 followed by secondary staining with Cy3-conjugated goat anti-rabbit immunoglobulin to visualize sc-894 labeling (red channel). (B) Quiescent SKBR-3 cells were stained against ErbB2 (with Alexa488-4D5, green channel) and against caveolin (as in part A, red channel). (C) SKBR-3 cells were treated with FITC-CTX-B for 30 minutes at 37°C (green channel), and then stained against ErbB2 (Cy5-4D5, blue channel) and against caveolin (as in part A, red channel). Bar, 1 µm.

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