Fig. 3. The EGTC is enriched in the markers expected for an ER-Golgi transport intermediate. Transmission electron micrographs of VSV-infected NRK cells subjected to the rapid ethane freezing procedure 10 minutes after release from 40°C. (A) Double immunogold labeling of VSVG (9 nm gold particles, small arrowheads) and COPI (ß-COP antibody, 14 nm gold particles, large arrowheads). Although found within the same structures (^*), VSVG labeling is more concentrated at the central matrix whereas ß-COP labeling (14 nm gold particles, arrowheads) is mostly associated with the bud profiles at the periphery. This is also illustrated in the single ß-COP labeling (10 nm gold particles, arrowheads) in B. This is also the case for p58 (p58 antibody, 10 nm gold particles, arrowheads) labeling as shown in C. Bars, 0.2 µm. (D) Double immunogold labeling for ß-COP (14 nm gold particles, large arrowheads) and the tether protein p115 (p115 antibody, 9 nm gold particles, small arrowheads), demonstrating that p115 is also found at a significant level in the EGTC. (E) Immunogold labeling for the soluble cargo albumin (albumin antibody, 10 nm gold particles) in HepG2 cells. Bars, 0.2 µm.

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