Fig. 1. Generation of Mat1 conditional (flox) mice. (A) Partial genomic structure of the murine Mat1 gene and a target vector used to generate the conditional targeted allele Mat1^flox. (B) Southern blotting analysis of targeted ES cell lines. SacI-digested genomic DNA from wild-type (wt) and targeted ES cell lines flox1 and flox2 yielding the predicted 9.3 kb band in addition to the 10.5 kb wild-type band. (C) PCR genotyping of the litter resulting from crossing Mat1^flox/+ males to Mat1^+/- females. A 385 bp wild-type band, a 477 bp conditional band, and a 310 bp null allele band are amplified with M10-M12 and M10-N4 primer pairs indicated in A. (D) PCR genotyping of control and experimental animals generated by crossing Mat1^flox/+;KCN males with Mat1^flox/flox females, with the Krox-20-Cre allele denoted KCN. The asterisk marks an introduced SacI site.

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