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Fig. 3. Targeted disruption of Mat1 in myelinated Schwann cells. (A) Indirect immunofluorescence detection of Krox-20 and Cre recombinase in longitudinal sections of sciatic nerves from control (Mat1flox/-) and mutant (Mat1flox/-;KCN) animals. Counterstaining of mutant sections was performed with Hoechst (H). Arrows point to non-targeted, Krox-20/Cre-negative cells. (B) Staining of control (Mat1flox/+;Z/AP) and mutant (Mat1flox/-;KCN;Z/AP) sciatic nerve sections for ß-galactosidase (lacZ) and alkaline phosphatase (AP). (C) Indirect immunofluorescence detection of Mat1 in control (Mat1flox/-) and mutant (Mat1flox/-;KCN) animals. Counterstaining was performed with Hoechst.





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