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Fig. 3. The effects of IGF-I on the actions of IGFBP-3 and -5 on the adhesion of Hs578T cells to ECM. The graphs show the percentage of cells attached. Cells were treated with (A) RGD (10 µg/ml), IGFBP-3 (100 ng/ml), IGF-I (50 ng/ml) or IGF-I and IGFBP-3 together or (B) RGD, IGFBP-5 (100 ng/ml), IGF-I or IGF-I and IGFBP-5 together. * indicates statistically significant differences as defined by Bonferroni/Dunn post-hoc test for RGD<CT; IGFBP-5>CT; IGFBP-3<CT; IGFBP-3+IGF-I>IGFBP-3; IGFBP-5+IGF-I<IGFBP-5. The data represent the mean of three experiments each in triplicate±s.e.m.





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