Fig. 4. The effects of an RGD-containing fibronectin fragment or soluble thrombospondin on the actions of IGFBP-3 and -5 on the attachment of Hs578T cells to ECM. The graphs show the percentage of cells attached. Cells were treated with either (A) RGD (10 µg/ml), IGFBP-3 (100 ng/ml) or IGFBP-3 and RGD, (B) RGD, IGFBP-5 (100 ng/ml) or IGFBP-5 and RGD, (C) RGD, IGFBP-3, IGFBP-5, Thrombospondin (TSP) (0.01 µg/ml), IGFBP-3 and TSP, or IGFBP-5 and TSP, or (D) RGD, IGFBP-5, TSP or IGFBP-5 and TSP where ^* RGD<CT, IGFBP-3<CT; IGFBP-5>CT, IGFBP-5+RGD<IGFBP-5 is considered statically significant as defined by Bonferroni/Dunn post-hoc test. Data represent the mean of three experiments each in triplicate±s.e.m.

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