Fig. 6. The effects of growing Hs578T cells on fibronectin on the ability of IGFBP-5 and -3 to modulate C2-induced death. The graphs show the percentage of dead cells. Cells were seeded on plastic (dark grey bars) or on fibronectin (light grey bars) coated wells for 24 hours prior to being switched to SFM for a further 24 hours before a pre-incubation with either (A) IGFBP-5 (100 ng/ml) or (B) IGFBP-3 (100 ng/ml) for 24 hours followed by a co-incubation with an apoptotic dose of ceramide. ^* is considered statically significant where on plastic C2>CT; IGFBP-5+C2<C2 and IGFBP-3+C2>C2, and on fibronectin IGFBP-5>CT, IGFBP-3+C2<C2. Data represent the mean of three experiments each in triplicate±s.e.m.

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