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Fig. 3. TRAF2 upregulation by RANKL is abolished in Jnk1-/- osteoclast progenitors. Purified BMMs were isolated and treated for 3 days in the absence (-) or presence (+) of RANKL (50 ng/ml). The expression of TRAF2 (A) or TRAF6 (B) protein was analyzed by western blotting. TRAF2 expression was also analyzed 1 hour following UV irradiation of JNK1-deficient BMMs (UV +). Loading was controlled by reblotting with an anti-{alpha} actin antibody. (C) The expression of TRAF2 mRNA was analyzed by northern blotting. RNA loading was controlled by probing with GAPDH.





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