Fig. 7. Cultures exposed to low serum fusing conditions were evaluated by immunofluorescence to determine the number of myogenic nuclei associated with fused and myosin-heavy-chain-expressing (MHC) myotubes at 96 hours (representative images of EP and LP CD34-positive populations are shown in A and B). Significant differences in the degree of fusion, defined by the fusion index (C), and ratio of mononuclear cells to myotubes formed (D) were observed between EP and LP populations (fusion index, ^*P<0.05 for CD34-positive vs. EP and CD34-negative; ratio of mononuclear to myotubes, ^*P<0.05 for CD34-positive vs. EP only).

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