Fig. 8. (A) Establishment of a CD34-positive subpopulation is observed when culturing CD34-depleted cultures under high serum culturing conditions. Cells enriched for CD34 expression maintain their phenotype over the same time period (representative dot plots are shown along with averaged percentages of CD34-negative and -positive cells at each time point). (B,C) Immunofluorescence and brightfield images of mononuclear myogenic cells found within cultures initially depleted of a CD34-expressing population and exposed to low serum fusion medium. The majority of the undifferentiated myogenic cells were found to express CD34 (B), although some CD34-negative cells are present (C, arrows; 20x objective).

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