Fig. 1. Chromosome missegregation in the ned1-1 mutant. (A) Diploid cells were incubated in a rich medium YPD at 36°C for indicated periods and plated on MR-plates at 26°C. Haploid (white in color) and aneuploid colonies (deep red, sectored) were scored. ^*For wild-type (WT), deep red colonies are categorized as aneuploid in the figure, however those with sectors were very rare, with undetermined genetic constituents. (B) FISH analysis of segregating chromosomes. Diploid cells were incubated in YPD medium at 36°C for 4 hours. Red, chromosome III (rDNA); green, chromosome II (cos713); blue, chromatin regions (DAPI). In the second panel, non-disjoined chromosome II is left in the mid-zone. In the bottom panel, one of the two chromosome III shows nondisjunction. Bar, 2 µm.

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