Fig. 2. Deformed nuclei and abnormal ER-like membranes in the ned1-1 mutant. (A) Haploid cells were incubated in YPD medium at 30°C. Merged images: blue, DAPI; red, ethidium bromide; green, MAb414. (B) Haploid cells are incubated in EMM2 medium at 30°C. The anti-Nup189 antibody was used instead of MAb414 (green), chromosomes were in red. (C) S. cerevisiae diploid nuclei stained with anti-Nup189. (D) Electron micrographs of diploid cells cultured at 36°C for 3 hours. N, nucleus; arrowheads, ER in wild-type; arrows, mesh-like (left bottom) and multi-layered structure (right) probably formed from enormously overdeveloped ER-like membranes in ned1-1 cells. (E) Haploid fission yeast cells transformed with pSec61-GFP. Cells are incubated in thiamine-free EMM at 30°C for 20 hours. Green, GFP; Red, DAPI. Bars, 2 µm.

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