Fig. 5. Genetic interactions of ned1-1 mutant with crm1, pim1 and spi1. (A) In the upper panels, indicated strains were incubated at 33°C for 3 days on YEA containing the indicated amounts of staurosporin (sta) or caffeine (caf). In the lower panel, the same strains were incubated on YEA at 24°C for 4 days. (B) Cell extracts prepared from the indicated strains were run on a 10% SDS-polyacrylamide gel and stained with Coomassie Brilliant Blue. The p25 band was only visible in the lane for crm1 single mutant. (C) Indicated strains were incubated at 33°C for 4 days. (D) The ned1-1 mutant cells were transformed with multicopy plasmids that carried the indicated gene. Transformants were plated on YEA containing 15 µg/ml TBZ, incubated at 30°C for 3 days.

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