QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?

The folly of phorbol ester

Activation of protein kinase C (PKC) by diacylglycerol generated through hydrolysis of phosphatidylinositol 4,5-bisphosphate is a classic signal transduction mechanism. Indeed, cell biologists and pharmacologists have over the years used the diacylglycerol analogue phorbol ester to implicate PKC in a wide variety of processes. Recent work, however, indicates that diacylglycerol and phorbol ester have additional targets, such as chimaerins, the Ras activator RasGRP and the vesicle-priming protein Munc-13. In a Commentary on p. 4399, Nils Brose and Christian Rosenmund review the evidence for such non-PKC targets. These proteins each contain the diacylglycerol-binding C₁ domain and function in processes in which PKCs were previously implicated. Release of neurotransmitter from hippocampal neurons, for example, is regulated by phorbol ester, but since neurons expressing a Munc-13 C₁-domain mutant cannot respond to phorbol ester the target must be Munc-13. Similar work indicates that RasGRP — and not PKC — couples T cell receptors to MAP kinase activation in thymocytes. Brose and Rosenmund conclude that susceptibility to phorbol esters does not alone constitute a test for PKC involvement and discuss additional criteria that should be satisfied.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in JCS:

Move over protein kinase C, you've got company: alternative cellular effectors of diacylglycerol and phorbol esters

Nils Brose and Christian Rosenmund
JCS 2002 115: 4399-4411. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?