Fig. 2. Disassembly of the Golgi apparatus is caused by G_z-selective RGSZ1 and RGSZ2, but not by other RGS proteins. (A) HeLa cells were transfected with a plasmid encoding N-terminal FLAG-tagged RGSZ1 (a,b), RGSZ2 (c,d), GAIP (e,f), RGS2 (g,h), RGS3 (i,j), or RGS4 (k,l). At 20 hours after transfection, the cells were fixed, and then double-stained with the anti-ß-COP antibody (a,c,e,g,i,k) and anti-FLAG antibody (b,d,f,h,j,l). Arrows indicate cells expressing RGS proteins. Bar, 20 µm. (B) The percentage of transfected HeLa cells with a dispersed ß-COP staining was determined. The results are expressed as the percentage of the number of cells with a dispersed Golgi apparatus and as the mean±s.e.m. for at least three separate experiments.

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