Fig. 7. Effects of the edible mushroom Agaricus bisporus lectin (ABL) on the serum-induced nuclear import of cPLA-. Cells were grown on coverslips and starved for 24 or 48 hours. Cells were then re-fed with complete medium or pre-treated with ABL (20 µg/ml) for 6 hours prior to the addition of complete medium. (A) cPLA- was detected by immunofluorescence microscopy. Bar, 10 µm. (B) Graph showing changes in intensity of nuclear staining (in arbitrary units, n=90, data represents averages from three independent experiments±s.e.m.) in sections taken through serum-starved, re-fed and ABL-treated re-fed cells.

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