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Fig. 5. Mutation of serine 345 to alanine or aspartic acid abolishes association of
Chk1 with 14-3-3 upon DNA damage. NW223 (Chk1), NW444 (S345A) and NW463
(S345D) were grown to mid-log phase, treated with CPT and lysed. Lysates were
incubated with antibody against Rad 24 (UMDNJ 55), subjected to SDS-PAGE and
immunoblotted with 12CA5 anti-HA antibody (IP). Control precipitations (NI)
with non-immune rabbit serum were processed exactly as anti-Rad24 IPs. Samples
of the lysates, before immunoprecipitation are shown in the left panel.
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