Fig. 3. Adhesion properties of mycelium from CBEL⁺ and CBEL^- P.p.n. strains growing on a cellophane membrane. The mycelium was grown on a cellophane membrane placed on top of V-8 agar medium. The membrane together with the mycelium was removed from the medium underneath (A-E), cut in sectors of similar sizes, which were then incubated for 2 hours under shaking in water. The presence of remaining adherent mycelium was checked by light microscopy (F-J). (A,F) Untransformed control strain; (B,G) EX11-1 CBEL⁺ strain; (C,H) EX11-4 CBEL^- strain; (D,I) EX3-5 CBEL⁺ strain; (E,J) EX3-3 CBEL^- strain. Results representative of three independent experiments are shown. The mycelial aggregates are indicated by arrows. Note the absence of residual mycelium in H and J. Bars, 70 µm.

Return to article