Fig. 4. The differentiation of lobed structures in mycelium from CBEL⁺ and CBEL^- P.p.n. strains in contact with a cellophane membrane. The mycelium was grown on a cellophane sheet placed on top of V-8 agar medium. The membrane with the mycelium was removed from the medium underneath, cut in sectors and observed under bright field microscopy. The whole membrane sector from each sample was screened, and typical data from triplicate samples are shown. (A,B) Lobed granular structures (arrows) from the EX11-1 (A) and EX3-5 (B) CBEL⁺ strains. The asterisk indicates an aggregate of intricate hyphae on a different focus plane. (C) Leakage of protoplasm (open arrow) from a damaged hyphae in the untransformed strain. (D) Hyphae from the EX11-4 CBEL^- strain. Note the absence of lobed structures. Bars, 40 µm.

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