Fig. 1. Genomic in situ hybridisation using total genomic rye DNA as a probe in wheat root sections followed by confocal microscopy imaged entire chromosomes during interphase. Projections of consecutive confocal sections of entire rye chromosome pairs are shown. (A) Two consecutive confocal sections of control seedlings germinated on water. The interphase chromosomes appear as elongated domains with the two arms very close together and only rarely distinguishable (arrows). Bar, 10 µm. (B,C) Stereo pairs of nuclei after germination in the presence of 5-AC. The two chromosome arms can be seen separated and the chromosome structure appears very irregular with a more complex and broken configuration. (D,E) Stereo pairs of nuclei after germination in the presence of TSA. Similar changes to those produced by 5-AC are seen. However, the overall Rabl configuration is maintained, with the centromeres and subtelomeric heterochromatic blocks (arrows in B,D) remaining at the nuclear periphery. Bar, 10 µm (B-E).

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