Fig. 2. Genomic in situ hybridisation in interphase nuclei from wheat root sections labelled an individual chromosome arm in the 1R wheat/rye translocation line. Projections of consecutive confocal sections are shown. The rye telomeric knobs (arrows in 2A) are clearly seen as intensely labelled regions at one end of each arm, located at the nuclear periphery (line in 2A). (A) A group of interphase nuclei from control seedlings germinated on water. A gap is usually seen at the position of the decondensed nucleolar organisers (NOR), where the rDNA genes are decondensed into the nucleolus (n). Bar, 10 µm. (B,C) Two nuclei from control seedlings. (D,E) Two nuclei after treatment with 5-AC. The chromosome arms are much more irregular, and regions of strong labelling are interrupted by several gaps of decondensed chromatin. (F,G) Two nuclei after treatment with TSA. Again the chromosome arms are interrupted by four to five gaps, but the overall configuration appears more regular than after 5-AC. Bar, 10 µm, in B-G.

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