Fig. 3. Effect of TSA on histone acetylation. Total extracted proteins from roots germinated in water (lane 1), in 15 µM TSA changed daily (lane 2) or in 15 µM TSA without changes (lane 3) were electrophoresed on a 15% SDS gel. (A) Total proteins stained with Coomassie, showing equivalent loading in the three lanes. (B) The equivalent gel to A was western blotted with antibody AHP 418, specific to acetylated histone H4. (C) Equivalent gel to A, western blotted with antibody AHP 416, specific to histone H4 acetylated at lysine 12. There is an increase in the labelling of the histone H4 band at 10 kDa in lanes 2 and 3 with both antibodies (arrows in B,C), showing increases in the amount of acetylated histone H4, but no difference in intensity between daily changes (lane 2) and a single application of TSA (lane 3).

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