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Fig. 3. Cells that express markers characteristic for hypertrophic and/or calcifying chondrocytes differentiated in EB outgrowths. Collagen X mRNA (A,B), osteopontin protein (C,D) and bone sialoprotein I + II (E,F) were expressed in cells organized in nodules (A,C,E) or as single cell clusters (B,D,F) in EB outgrowths. Representative areas of EB outgrowths at 5+23 d (A), 5+16 d (B) and 5+30 d (C-F) are shown. Bar, 100 µm.





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