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Fig. 3. Phenotype of Rho3Q71L-overexpressing cells. (A) Overexpression of Rho3Q71L produced large dumpy cells and mini-cells. Wild-type cells carrying pREP1Rho3Q71L were grown at 30°C in EMM without thiamine for 18 or 24 hours. DIC images are shown. (B) F-actin ring was abnormally formed in Rho3Q71L-overexpressing cells. The cells (18 hours in A) were fixed and stained with Rhodamine-phalloidin (top) and DAPI (bottom). Deconvoluted 3D images are shown. Bars, 10 µm. (C) Electron micrographs of Rho3Q71L-overexpressing cells (18 hours in A). Arrowheads indicate abnormally formed septa. N, nucleus. Mt, mitochondria. Bar, 0.2 µm.





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