Fig. 2. FasL induced both apoptotic and necrotic cell death in A20 B-lymphoma cells. Cells were incubated for 30 minutes with or without 100 µM Ac-DEVD-cho (DEVD) or 10 µM zVAD-fmk (zVAD). Then FasL was added to a final concentration of 100 ng/ml, and cells were incubated for another 16 hours. Subsequently, cells were stained with PI, and cell survival was determined by flow cytometric analysis. (A) In response to FasL, two populations of PI-positive, dead cells, indicated as M1 (hypodiploid population) and M2 (dead cells with intact DNA), were distinguishable. (B) Statistical analysis of results from experiments as indicated in A, showing the means with standard deviation of three independent experiments. As additional negative controls, results obtained with cells resistant to FasL (A20R) are also shown. (C) In parallel, cells analyzed as in A were permeabilized with methanol and stained with PI to quantify total DNA content. Data shown are representative of three independent experiments with similar outcomes.

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