Fig. 5. Comparison of FasL-induced cell death in A20, Jurkat and Raji cells. (A) Cells were incubated either with increasing concentrations up to 400 ng/ml of FasL (A20, Jurkat cells) or up to 400 ng/ml of anti-Fas antibody (Raji cells) for 16 hours, stained with PI and cell death was assessed. (B) A20 and Jurkat cells were incubated with or without (NT) 100 ng/ml FasL after pre-treatment for 30 minutes with the indicated low concentrations (0-5 µM) of zVAD-fmk (zVAD). Cell death was quantified by flow cytometric analysis (see Fig. 2). Squares or empty bars indicate the percentage of apoptotic cells, and circles or gray bars indicate necrotic cells (A,B). Results averaged from three experiments are shown. Also, PS exposure on the cell surface of A20, Jurkat and Raji cells was detected by FACS using Annexin-V-FITC 5 hours following Fas activation (C). Subpopulations R1 and R2 indicated for A20 and Jurkat cells were obtained as described in Fig. 4. Results shown are representative of two independent experiments.

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