Fig. 4. Association of Ran with chromosomes in a cultured mammalian cell. A mitotic NRK cell was injected with Alexa 488 Ran and 70 kDa Rh dextran. (A) The position of the chromosomes is seen in a scanning transmission light image. (B) 70 kDa Rh dextran is excluded from the region of the chromosomes. (C) Fluorescent Ran is present throughout the spindle region, and in particular, in the region of the chromosomes. Since a non-interacting molecule should have a similar distribution to the fluorescent dextran, this is evidence for a Ran association with chromosomes. Bar, 10 µm.

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