Fig. 1. Centrosomin localizes to centrosomes and to extracentrosomal particles. The localization of Cnn in early syncytial embryos was determined by immunostaining with antibodies directed against Cnn. The image of the specimen in A was acquired with a low signal gain, showing Cnn localization at the centrosome to have a doughnut-like appearance. In contrast to A, the specimen in B was acquired at a high signal gain, showing the presence of Cnn-containing extracentrosomal particles around the centrosomes. (C) An electron micrograph of an embryonic centrosome at interphase of cycle 14 that has been immunogold labeled for Cnn. The image shows that Cnn is a component of the electron-dense pericentriolar material, the tentacle-like projections of which are enriched in Cnn. The Cnn signal appears as black dots, some of which are indicated with white arrows, that are more concentrated within the PCM. See also Movie 1, available at jcs.biologists.org/supplemental.

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