Fig. 5. Cnn and D-TACC colocalize at flare particles. Shown is a wild-type (OreR) interphase syncytial stage embryo double immunostained for D-TACC (green) and Cnn (red). DNA, stained with TOTO-3, is shown in blue. Yellow arrows indicate several flare particles in the D-TACC, Cnn and the merged images. Not all Cnn-immunoreactive particles contained D-TACC and vice versa. In the merged image the D-TACC signal at the centrosome appears to extend further from the center than does Cnn, but this is an inaccurate representation of centrosome structure and is due to an higher gain placed on the D-TACC signal at data collection, which was necessary to bring the flare particle signal to a similar level to that achieved with Cnn. See also Movie 4 at jcs.biologists.org/supplemental.

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