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Fig. 8. Flare particles are restricted by the actin cage. Rhodamine-actin was injected into GFP-Cnn embryos to view the dynamics of the centrosomes and the actin cytoskeleton live. In metaphase (030), the flare particles, indicated by yellow arrows, moved to the actin cage boundary and no further. At telophase, when the centrosomes approach each other in close proximity (297), actin assembled densely at the centrosomes (see arrows) on the sides opposite to the nucleus. Following division, actin caps formed over the centrosomes (474) and in interphase the actin cage bound the flares (594). See also Movie 6 at jcs.biologist.org/supplemental.





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