Fig. 7. Distribution of histone H4 acetylation at lysine 12 during S. ocellaris spermiogenesis (A-C) and in an embryo at early fertilization stage (D). DAPI staining of elongated spermatids (A), late spermatids (B), mature spermatozoa (C) and the whole embryo (D). (A',B',D') Indirect immunolabeling with H4Ac12 antibody and (A'',B'',D'') superimposed images where antibody staining is in red. In A',A'' H4Ac12 distributes on the nuclear chromatin whereas in B',B'' the labeling is restricted to a few nuclear sites. In C no labeling is observed. In D the short arrow points to the sperm nucleus and long arrows to the haploid maternal meiotic products. (D',D'') Enlarged images of the sperm nucleus and maternal chromosomes where it is seen that H4Ac12-labelling localizes to the decondensing sperm nucleus chromatin and not to the maternal chromatin. Bar, 10 µm.

Return to article