Fig. 4. Comparison of the effects of Wnts on the initiation of chondrogenic differentiation of stage 23/24 wing mesenchymal cells in micromass cultures after three days. (A) RT-PCR analysis of retroviral transgene expression. (B) Quantification of alcian blue staining (solid bars) and nodule numbers (open bars). (C) Micromass cultures stained for cartilaginous matrix with alcian blue. In B, values shown are the mean+s.d. of 11 cultures from three independent experiments. ^*P<0.0001; ^**P<0.0005; ^***P<0.005 (Student's t-test) of Wnt/Ihh-infected cultures compared to control cultures.

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