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Fig. 8. Immunochemical analysis of CgA domain-GFP fusion protein release upon
stimulation of regulated secretion. PC12 cells were transiently transfected
with expression plasmids encoding the GFP fusion proteins indicated. 48 hours
after transfection, regulated secretion was triggered by 2 mM BaCl2
for 15 minutes. Extracellular media were collected and concentrated using C-18
SepPak cartridges, and processed for immunoblot using an anti-GFP antibody.
Immunoreactivity was visualized by chemiluminescence.
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