Fig. 4. Localization of proteins involved in membrane events on actin individualization complexes. Confocal images of individualizing spermatids double-labeled with Alexa Fluor 488 phalloidin (A,G,J) and anti-dynamin antibody (B), anti- adaptin antibody (H) or anti-amphiphysin antibody (K). In double labeled samples, myosin VI (E) was concentrated at the front of dynamin-stained complexes (D). Dynamin and amphiphysin colocalized with actin in the individualization complexes (C,L); however, unlike dynamin, amphiphysin also was concentrated at the front of the actin complexes.

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