Fig. 2. Neurite outgrowth on neutral or acidic laminin matrices. Dissociated cortical cells were incubated for 24 hours over laminin matrices obtained at neutral (A) or acid pH (B). Immunocytochemistry for neuronal cells was carried out using a polyclonal anti-Tau reagent as the primary antibody and Cy3-labeled anti-rabbit as the secondary antibody. Panels C-F show quantitative analyses of data obtained in panels A (pH 7, open bars) and B (pH 4, closed bars). Analyses were carried out considering only the 30 longest neurites (C) or 100 neurons randomly chosen (D-F). Three asterisks correspond to P<0.0001. Bar, 100 µm.

Return to article