Fig. 8. Effect of protein kinase inhibitors on neuritogenesis. Cortical cells were plated over laminin matrices polymerized under either neutral (A, C, E and G) or acidic conditions (B, D, F and H) and incubated for 24 hours in the absence of inhibitors (A and B) or in the presence of 0.1 µM H-89 (C and D) or 0.15 nM staurosporin (E and F). The scale bar corresponds to 100 µm. The lowest panels show average neurite lengths (quantified as the 30 longest neurites) in each case. Three asterisks correspond to P<0.001.

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