Fig. 1. Identification of the DOCK180 superfamily of proteins. (A) Phylogenetic tree of DOCK180-related proteins. Amino-acid sequences that cover the region of the DHR-2 domains (see below) of the indicated human, Drosophila and C. elegans family members were aligned with ClustalW. The tree was derived by neighbor-joining analysis applied to pairwise sequence distances calculated with the PHYLIP package using the Kimura two-parameter method to generate unrooted trees. The final output was generated with TREEVIEW. The number at each node represents the percentage of bootstrap replicates (out of 100). The four subfamilies of DOCK180-related proteins (DOCK-A, -B, -C and -D) are indicated. (B) Schematic diagram of the structure of representative members of the human DOCK180 superfamily. Percentage identity between the various SH3, DHR-1 and DHR-2 domains in amino-acid sequence to DOCK180 is indicated. Structures of DOCK180, DOCK2, DOCK3 and DOCK9 were derived from full-length sequences. The sequence for DOCK6 could be truncated at the N-terminus (indicated by a yellow dashed line).

Return to article