|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 3. Coexpression of ELMO1 is not required for DOCK180-mediated Rac activation
in LR73 cells. (A) LR73 cells were transfected with a control plasmid or with
the indicated amounts of plasmids coding for Flag-DOCK180 and Myc-ELMO1. GTP
loading of Rac was measured by the PBD pull-down assay, and the precipitated
Rac was analyzed by immunoblotting with anti-Rac antibodies. Expression levels
of Rac, Flag-DOCK180 and Myc-ELMO1 were analyzed by anti-Rac, anti-Flag and
anti-Myc immunoblotting, respectively, in total cell lysates. (B) LR73 cells
were transfected with a control plasmid or with the indicated amounts of
plasmids coding for Flag-DOCK180 with or without cotransfection of 1 µg of
ELMO1-GFP. GTP loading of Rac was measured by the PBD pull-down assay, and the
precipitated Rac was analyzed by immunoblotting with anti-Rac antibodies.
Expression levels of Rac, Flag-DOCK180 and ELMO1-GFP were analyzed by
anti-Rac, anti-Flag and anti-GFP immunoblotting, respectively, in total cell
lysates.
|
