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Fig. 6. DRAL/FHL-2 interacts with the metabolic enzymes MM-CK, adenylate kinase and phosphofructokinase. (A) Transient transfection assays. GFP-tagged MM-CK (a), phosphofructokinase (b), and adenylate kinase (c) colocalise with DRAL/FHL-2-FLAG (a', b', c', d') in neonatal rat cardiomyocytes, with a strong signal around the Z-disc (arrowheads) and a weaker signal in the M-band (arrows). GFP-tagged Smpx/Csl (d) is also colocalised with DRAL/FHL-2. (B) Interaction of the metabolic enzymes with DRAL/FHL-2 in pull-down assays. MM-CK, adenlyate kinase and phosphofructokinase interact with DRAL/FHL-2-GST, but not with GST alone. Despite its similar localisation pattern in cardiomyocytes, Smpx/Csl does not interact with DRAL/FHL-2 in the pull-down assay. PFK, phosphofructokinase; AK1, adenylate kinase. Bar, 10 µm.





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