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Fig. 7. DRAL/FHL-2 interacts with the four and a half LIM domain protein FHL-1 but not with the LIM-only protein MLP. Pull-down assays: FLAG-tagged FHL-1 interacts with DRAL/FHL-2-GST but not with GST alone. In contrast, HA-tagged MLP shows no interaction with either GST-DRAL/FHL-2 or GST alone. The interaction of FHL-1 with DRAL/FHL-2 can be abolished by the addition of 10 mM EDTA, which unfolds LIM domains through the removal of the coordinated metal ions.





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