Fig. 7. Transfection of hNFL does not affect the microtubule network of SW13 Vim^- cells. Transient transfection of wild-type hNFL (A), D469N variant hNFL (B), P8R mutant hNFL (C) and Q333P mutant hNFL (D) into SW13 Vim^- cells. Cells were co-stained with monoclonal anti-ß-tubulin antibody (green) and polyclonal anti-NFL antibody (red). Shown are the merged images corresponding to staining with both antibodies. Transfection of hNFL constructs did not affect the microtubule network, and NFL and tubulin did not colocalize.

Return to article