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Fig. 3. Levels of Kar2p in Y. lipolytica wild-type and {Delta}sbh1 strains treated with tunicamycine. Exponential cultures were treated for 3 hours with 10 µg/ml tunicamycine before intracellular protein extraction. Same amounts of total protein were separated by SDS-PAGE, transferred onto nitrocellulose and blotted using anti-kar2p antibodies.





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