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Fig. 4. Intracellular level of precursor forms of the alkaline extracellular protease in Y. lipolytica wild-type and {Delta}sbh1 strains. Total intracellular protein was extracted from wild-type cells (lane 1), wild-type cells transformed with the mutated copy of the XPR2 gene (lanes 2 and 3), {Delta}sbh1 cells (lane 4) and {Delta}sbh1 cells expressing the mutant protease (lanes 5 and 6). Proteins were analyzed by SDS-PAGE and blotted with anti-AEP antibodies.





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