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Fig. 7. Analysis of the interacting domain of Sbh1p with Cnx1p using the two-hybrid system. All diploids are able to grow on medium A, and only interaction between Sbh1p and Cnx1p allows growth on medium B (expression of two reporter genes is required). 1 and 2, pAS2{Delta}{Delta}SBH1 + pGADCNX1 (entire protein); 3, pAS2{Delta}{Delta}SBH1{Delta}SalI (deletion of the C-terminal domain) + pGADCNX1; 4, pAS2{Delta}{Delta} + pGADCNX1.





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