Fig. 5. Complementation analysis of Yck2p C-terminal variants. (A) Galactose-inducible plasmids were introduced into yck^ts strain LRB951 and cultures were grown in selective medium containing 2% raffinose as the carbon source, and then plated onto medium containing 2% galactose. (B) Plasmids encoding the indicated Yck2p variants were introduced into the yck^ts strain LRB951. Transformants were grown to mid-log phase inmedia selective for plasmid retention and 5 µl drops of each culture and of 1/10 diluted cultures were plated onto selective agar media at the indicated temperature. Plates were incubated for 24 (glucose) or 48 (galactose) hours at 24°C or at 37°C before photographing.

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