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Fig. 8. GFP-yck2{Delta}519-527p fails to accumulate to normal levels. Immunoblot showing steady-state GFP-Yck2p, GFP-yck2-C545,546Sp and GFP-yck2{Delta}519-527p levels in wild-type (LRB906) cells transformed with low-copy plasmids encoding the indicated fusion proteins. Protein samples were separated and transferred to nitrocellulose as described in Materials and Methods. Membranes were probed independently with {alpha}GFP and {alpha}PGK antibodies.





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