Fig. 9. A GFP-Yck2/Yck1p chimera is modified and targeted to the plasma membrane similarly to GFP-Yck2p. (A) Sequence alignment of Yck2p and Yck1p C-terminal sequences. The sequences swapped in the Yck2/Yck1p chimera are shown. (B,C) Plasmid pL240, expressing GFP-Yck2/Yck1p upon galactose induction, was introduced into wild-type strain LRB906 and akr1/akr1 strain LZY103 (B) and into sec9, sec14 and sec23 strains (C). (B) Cells were grown to log phase in selective medium containing 2% raffinose as the carbon source, then galactose was added to 2% and cells were incubated at 30°C for 120 minutes before photographing. (C) Cells were grown and treated as described for Fig. 2 except that the induction period was 120 minutes. Bar, 2 µm.

Return to article