Fig. 2. Lte1 cortex association is largely independent of an intact secretion pathway but responds to defects in septin structure. (A,B) Wild-type and sec18-1 (SY132) cells were shifted to 37°C for 90 minutes and Lte1 localisation was observed and quantified. Bar, 10 µm. (C) Lte1GFP localisation in wild-type and cdc12-6 (SY134) mutant cells at permissive temperature and following 20 minutes shift to 37°C. (D) Introduction of CDC12, but not plasmid alone, restores Lte1 to the cortex (a-b). (E) Phosphorylation status/stability of Lte1 is not affected by septin mutation. Wild-type and cdc12-6 (SY133) strains expressing HA3 tagged Lte1 were arrested with nocodazole at 20°C. Cultures were shifted 20 minutes to 37°C and cells collected for protein analysis.

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