Fig. 4. Cdc14 activity induces dissociation of Lte1 from the cortex. (A) A strain carrying GAL1-inducible CDC14 (SY140) was grown in YPRaffinose and arrested with nocodazole. At the arrest either 2% galactose (+) or 2% dextrose (-) was added. At the indicated times Lte1GFP fluorescence was monitored. Representative cells are shown below the table. Bar, 10 µm. (B) A strain harbouring a GAL1-inducible SIC1 plasmid (SY139) was treated as described in A. Cells were quantified in a similar fashion. A multibudded phenotype was observed upon extensive SIC1 overexpression but pictures are representative of cells at the 2 hour time point.

Return to article